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Published on: October 8, 2015
[Expression of PEDF mRNA in non-small cell lung cancer by real-time PCR method]
Lijian Zhang1, Jinfeng Chen, Yang Ke
1Surgery Department, Peking University School of Oncology, Beijing Cancer Hospital, Beijing 100036, P.R.China.
Background:
SYBR Green I is a non-probe real-time PCR method. It is quite convenient and its specificity is good. The aim of this study is to establish a quantitative SYBR Green I real-time PCR method for detection of PEDF gene in non-small cell lung cancer (NSCLC), and to investigate the relationship between PEDF mRNA expression and the clinicopathological characteristics.
Methods:
The target segments of PEDF and GAPDH proliferated by RT-PCR were diluted and used as the standard templates. PEDF mRNA was detected in 21 NSCLC tissues and matched paracancerous pulmonary tissues by relative quantitative method.
Results:
All the lung cancer tissues and the matched paracancerous pulmonary tissues had expression of PEDF mRNA. The relative level of PEDF mRNA expression was 0.5505±0.3590 (0.11-1.11) in the lung cancer tissues and 0.7219±0.2582 (0.29-1.31) in the matched paracancerous pulmonary tissues respectively (P=0.024). PEDF expression was significantly related to TNM stages (I-II vs III-IV, P=0.010) and the tumor size (T1 vs T2-4, P=0.007).
Conclusions:
The established SYBR Green I quantitative real-time PCR method can successfully detect the expression of PEDF mRNA. The primary results show that PEDF may be a protective factor in oncogenesis and development of NSCLC.
