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Related Concept Videos

Chromosome Replication02:31

Chromosome Replication

Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin of...
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DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
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In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
DNA Damage Can Stall the Cell Cycle02:36

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The process of chromosome duplication during cell division requires genome-wide disruption and re-assembly of chromatin. The chromatin structure must be accurately inherited, reassembled, and maintained in the daughter cells to ensure lineage propagation.
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Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique
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Histone demethylation and timely DNA replication.

Erica L Gerace1, Danesh Moazed

  • 1Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.

Molecular Cell
|December 15, 2010
PubMed
Summary
This summary is machine-generated.

Histone demethylases of the JMJD2 family promote DNA replication in silent genome regions. This process is crucial for late-replicating heterochromatin containing H3K9 methylation and HP1.

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Last Updated: Jun 6, 2026

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09:14

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Area of Science:

  • Epigenetics and Genome Regulation
  • Molecular Biology
  • Chromatin Biology

Background:

  • Silent genomic regions are known to replicate late in S phase.
  • Histone modifications play a critical role in regulating DNA replication timing.
  • Heterochromatin, characterized by H3K9 methylation and HP1 proteins, is typically late-replicating.

Discussion:

  • Black et al. (2010) identify a conserved function for JMJD2 histone demethylases.
  • These enzymes are implicated in facilitating replication within specific silent chromatin domains.
  • The study links JMJD2 activity to regions marked by H3K9 methylation and HP1.

Key Insights:

  • JMJD2 family histone demethylases promote replication in silent chromatin.
  • This function is conserved across species.
  • The activity of JMJD2 is specifically linked to heterochromatin marked by H3K9me and HP1.

Outlook:

  • Further investigation into the precise mechanisms by which JMJD2s influence replication fork progression in heterochromatin.
  • Exploring the therapeutic potential of targeting JMJD2s for diseases associated with replication timing defects.
  • Understanding the interplay between histone demethylation and heterochromatin formation/maintenance during DNA replication.