Related Experiment Video
Updated: Jun 6, 2026

Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels
Published on: December 13, 2024
Intracellular calcium level is upregulated by interleukin-32 in auditory cells
Hyun-Ja Jeong1, Na-Ra Han, Phil-Dong Moon
1Biochip Research Center, Hoseo University, 165, Sechul-ri, Baebang-myun, Asan, Chungnam 336-795, Republic of Korea.
Abstract:
Interleukin (IL)-32 has been associated with inflammation, apoptosis, and chemokine induction. The intracellular Ca(2+) concentration of mammalian endolymph in the inner ear is required for normal hearing and balance. Here, we document for the first time that IL-32 highly increased intracellular calcium level and IL-1β expression in an auditory cell line, HEI-OC1 cells. Treatment with 1, 2-bis (2-aminophenoxy) ethane-N, N, N', N'-tetraacetic acid acetoxymethyl ester, a chelator of intracellular calcium, inhibited IL-32-induced IL-1 β production and caspase-1 activation. Thus, IL-32 may contribute to modulation of the inflammatory reaction through the regulation of intracellular Ca(2+) in the inner ear.
Related Concept Videos
Feedback Regulation of Calcium Concentration
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
Calmodulin-dependent Signaling
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
Hair Cells
The Cochlea
Auditory Pathway
When viewed cross-sectionally, the cochlea reveals the scala vestibuli and scala tympani flanking the...
Amplifying Signals via Second Messengers

