Related Experiment Video
Updated: Jun 6, 2026

Generation of a Mouse Spontaneous Autoimmune Thyroiditis Model
Published on: March 17, 2023
Decreased CD4+CD152+ T cell subset and its correlation with the level of antithyroid antibodies in children with
Anna M Kucharska1, E Gorska, M Wasik
1Klinika Pediatrii i Endokrynologii, Warszawski Uniwersytet Medyczny, Warszawa, Poland. anna.kucharska@litewska.edu.pl
Objective:
Cytotoxic T lymphocyte antigen-4 (CTLA-4) is one of the basic antigens involved in immune responses regulation associated with autoimmune thyroid diseases. The aim of the study was to evaluate whether the surface expression of CTLA-4(CD152) on T cells is correlated with laboratory autoimmune markers in children with Hashimoto's disease.
Material And Methods:
Blood samples were obtained from 45 children with Hashimoto's thyroiditis of the mean age 14.8 ±2.35 years, and from 55 healthy age-matched children, free of allergic, immune and hematological disorders, and with a normal thyroid function. The anti-thyroid antibodies were measured with Microparticle Enzyme Immunoassay (AxSYM Anti-Tg, AxSYM Anti-TPO). The T cell phenotype was evaluated flow cytometery, with the use of monoclonal antibodies combination: CD4- FITC/ CD28 -PC5/ CD152 -PE and CD8 -FITC/ CD28 -PC5/ CD152 -PE. -
Results:
The percentage of T cells with CD152 expression was significantly decreased in children with Hashimoto's thyroiditis compared with healthy controls (P<0.001). A significant negative correlation was found between the level of anti-thyroglobulin antibodies and the percentage of CD4+CD152+ T cells (r = -0.34; P<0.05). Anti-thyroperoxidase antibodies did not correlate with CD152 expression.
Conclusions:
In children with Hashimoto's thyroiditis, the number of CD4+CD152+ T cells is decreased and negatively correlates with the level of anti-thyroglobulin antibodies.
Related Concept Videos
Synthesis and Regulation of Thyroid Hormones
Upon reaching the thyroid gland, TSH stimulates the follicular cells' active uptake of iodide ions from the blood. The ions diffuse to the apical surface of the cells and are oxidized to iodine. The iodine is then...
T Cell Types and Functions
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Graves' Disease I: Introduction
Hyperthyroidism II: Pathophysiology
Graves Disease II: Pathophysiology
T Cell Activation and Clonal Selection
Naive T cells that have not yet encountered an antigen express two primary CD...
