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Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Related Experiment Video

Updated: Jun 6, 2026

Quantification of Endosome and Lysosome Motilities in Cultured Neurons Using Fluorescent Probes

Quantification of Endosome and Lysosome Motilities in Cultured Neurons Using Fluorescent Probes

Published on: May 22, 2017

Quantifying translational mobility in neurons: comparison between current optical techniques

Sally A Kim¹, Hugo Sanabria, Michelle A Digman

¹Department of Neurobiology and Anatomy, University of Texas Health Science Center at Houston, Houston, Texas 77030, USA.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience

|December 15, 2010

Summary

No abstract available in PubMed .

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