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Fixation and Sectioning

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...

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Related Experiment Video

Updated: Jun 6, 2026

Simultaneous ex vivo Functional Testing of Two Retinas by in vivo Electroretinogram System

Simultaneous ex vivo Functional Testing of Two Retinas by in vivo Electroretinogram System

Published on: May 6, 2015

How to perform the reversed fixation test.

Michael C Brodsky, Katherine J Fray

The American Orthoptic Journal

|December 15, 2010

Summary

This summary is machine-generated.

The reversed fixation test aids in diagnosing subtle eye alignment issues like dissociated vertical divergence and horizontal deviation. This technique is valuable for complex strabismus cases.

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Optimized Protocol for Retinal Wholemount Preparation for Imaging and Immunohistochemistry

Optimized Protocol for Retinal Wholemount Preparation for Imaging and Immunohistochemistry

Published on: December 13, 2013

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Last Updated: Jun 6, 2026

Simultaneous ex vivo Functional Testing of Two Retinas by in vivo Electroretinogram System

Simultaneous ex vivo Functional Testing of Two Retinas by in vivo Electroretinogram System

Published on: May 6, 2015

Optimized Protocol for Retinal Wholemount Preparation for Imaging and Immunohistochemistry

Optimized Protocol for Retinal Wholemount Preparation for Imaging and Immunohistochemistry

Published on: December 13, 2013

Area of Science:

Ophthalmology
Clinical Neurology

Background:

Dissociated deviations, including dissociated vertical divergence (DVD) and dissociated horizontal deviation (DHD), present diagnostic challenges in complex strabismus.
Subtle forms of these deviations can be difficult to detect with standard clinical examinations.

Purpose of the Study:

To describe the technique for performing and interpreting the reversed fixation test.
To highlight the utility of the reversed fixation test in diagnosing subtle dissociated deviations.

Main Methods:

The reversed fixation test involves specific eye movements and observation protocols.
Detailed description of the procedure for eliciting and observing the deviation.

Main Results:

The reversed fixation test effectively visualizes subtle dissociated vertical divergence.
The test is also useful for diagnosing dissociated horizontal deviation in complex strabismus.

Conclusions:

The reversed fixation test is a simple yet powerful clinical tool for diagnosing subtle dissociated deviations.
This technique enhances the diagnostic capabilities for patients with complex strabismus.