Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Arousal state modulates human hippocampal ripples.

bioRxiv : the preprint server for biology·2026
Same author

Target RNA-triggered CRISPR-Cas12a2 preferentially cleaves collateral DNA over RNA.

Nucleic acids research·2026
Same author

YprA-family helicases provide the missing link between diverse prokaryotic immune systems.

Cell host & microbe·2026
Same author

Target RNA-triggered CRISPR-Cas12a2 Preferentially Cleaves Collateral DNA over RNA.

bioRxiv : the preprint server for biology·2026
Same author

Armored chimeric antigen receptor T-cell therapy targets antigen-heterogeneous glioma.

Cancer research·2026
Same author

Guide DNA - not RNA - expands the CRISPR toolkit.

Nature biotechnology·2026
Same journal

Correction to 'scSuperAnnotator: A platform for benchmarking comparison and visualizing automated cellular annotation methods for scRNA-seq data'.

Nucleic acids research·2026
Same journal

Correction to 'Differentiable partition function calculation for RNA'.

Nucleic acids research·2026
Same journal

Deployment of non-canonical splicing in tunicate genomes is mediated by divergent U2AF function and changing m6A modification in U1 and U6 snRNA.

Nucleic acids research·2026
Same journal

Bacillus subtilis DnaB forms multiple protein-protein interactions essential for DNA replication initiation.

Nucleic acids research·2026
Same journal

Multiple forms of protein-protein and DNA binding are exhibited by BrxC from the BREX phage restriction system.

Nucleic acids research·2026
Same journal

Biosynthesis of glycosylated 5-hydroxycytosine in the DNA of diverse viruses.

Nucleic acids research·2026
See all related articles

Related Experiment Video

Updated: Jun 6, 2026

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools
09:29

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

Published on: August 21, 2019

Design of small molecule-responsive microRNAs based on structural requirements for Drosha processing.

Chase L Beisel1, Yvonne Y Chen, Stephanie J Culler

  • 1Division of Chemistry and Chemical Engineering, 1200 E. California Blvd., MC 210-41, California Institute of Technology, Pasadena, CA 91125, USA.

Nucleic Acids Research
|December 15, 2010
PubMed
Summary
This summary is machine-generated.

Researchers engineered ligand-responsive microRNAs (miRNAs) for precise gene silencing control. This breakthrough enables tunable gene regulation by integrating aptamers into miRNA structures, advancing synthetic biology applications.

More Related Videos

Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes
11:58

Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes

Published on: January 30, 2019

MicroRNA-based Regulation of Picornavirus Tropism
09:05

MicroRNA-based Regulation of Picornavirus Tropism

Published on: February 6, 2017

Related Experiment Videos

Last Updated: Jun 6, 2026

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools
09:29

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

Published on: August 21, 2019

Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes
11:58

Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes

Published on: January 30, 2019

MicroRNA-based Regulation of Picornavirus Tropism
09:05

MicroRNA-based Regulation of Picornavirus Tropism

Published on: February 6, 2017

Area of Science:

  • Molecular Biology
  • Synthetic Biology
  • RNA Biology

Background:

  • MicroRNAs (miRNAs) are crucial regulatory RNAs involved in gene silencing and cellular processes.
  • Developing synthetic RNA-based systems is hindered by limited understanding of miRNA structure-function relationships.
  • Existing systems lack fine-tuned control over miRNA-mediated gene silencing.

Purpose of the Study:

  • To design novel ligand-responsive microRNAs (miRNAs) for controllable gene silencing.
  • To establish a strategy for integrating sensing and regulatory functions into synthetic miRNAs.
  • To enable titratable control over gene silencing based on user-defined inputs.

Main Methods:

  • Leveraged the relationship between Drosha processing and miRNA basal segment structure.
  • Integrated aptamers into the miRNA basal segments to create ligand-binding sites.
  • Demonstrated inhibition of Drosha processing upon ligand binding to aptamers.
  • Validated the strategy using three distinct aptamer-small molecule ligand pairs.

Main Results:

  • Ligand binding to integrated aptamers successfully inhibited Drosha processing.
  • This inhibition resulted in titratable control over gene silencing.
  • The control strategy proved general across different aptamer-ligand pairs.
  • The platform demonstrated potential for designing miRNA clusters and self-targeting miRNAs.

Conclusions:

  • Developed a versatile platform for engineering ligand-responsive miRNAs.
  • Enabled tunable gene silencing through user-defined small molecule inputs.
  • The platform facilitates fine-tuning of regulatory strength and dynamics for synthetic gene circuits.
  • Advances applications in biological research and medicine through precise RNA-based control.