Related Experiment Video
Updated: Jun 6, 2026
07:19
Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy (ATOM)
Published on: June 28, 2017
Design of a novel flow-and-shoot microbeam.
1RARAF, Columbia University, 136 S. Broadway, Irvington, NY 10533, USA. gyg2101@columbia.edu
Radiation Protection Dosimetry
|December 15, 2010
Summary
A new Flow-And-ShooT (FAST) microbeam system enables high-throughput cell irradiation. This technology significantly increases experimental speed and allows for the analysis of non-adherent cells.
Area of Science:
- Biophysics
- Cell Biology
- Microfluidics
Background:
- Microbeam irradiation is crucial for cellular studies.
- Current methods face limitations in throughput and cell type applicability.
Purpose of the Study:
- To introduce the novel Flow-And-ShooT (FAST) microbeam technology.
- To demonstrate its capability for high-throughput and non-adherent cell irradiation.
Main Methods:
- Cells are transported fluidically through a microfluidic channel intersecting the microbeam path.
- Real-time imaging and tracking are performed using a high-speed camera.
- A magnetic Point and Shoot system dynamically targets cells for irradiation.
Main Results:
- The FAST system is designed for controlled fluidic cell transport and real-time targeting.
- Initial tests show successful imaging and tracking of cells.
- The system is projected to achieve a throughput of 100,000 cells per hour.
Conclusions:
- The FAST microbeam technology offers a significant advancement in experimental throughput.
- It enables the irradiation of previously challenging non-adherent cell types.
- This innovation is expected to benefit a wide range of RARAF users.
