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Updated: Jun 6, 2026
07:48
An Orthotopic Bladder Cancer Model for Gene Delivery Studies
Published on: December 1, 2013
Two survivin polymorphisms are cooperatively associated with bladder cancer susceptibility
Naoko Kawata1, Norihiko Tsuchiya, Yohei Horikawa
1Department of Urology, Akita University Graduate School of Medicine, Akita, Japan.
International Journal of Cancer
|December 15, 2010
Summary
Two survivin gene single-nucleotide polymorphisms (SNPs) influence bladder cancer risk. The C-31G SNP increases risk, while the A9194G SNP decreases it, with both SNPs cooperating to affect susceptibility.
Area of Science:
- Genetics
- Oncology
- Molecular Biology
Background:
- Abnormal survivin expression is linked to various cancers.
- The influence of survivin promoter C-31G and exon 4 A9194G single-nucleotide polymorphisms (SNPs) on cancer susceptibility requires further investigation.
Purpose of the Study:
- To investigate the association between survivin C-31G and A9194G SNPs and bladder cancer susceptibility and progression.
Main Methods:
- Genotyping of 235 bladder cancer patients and 346 healthy controls for C-31G and A9194G SNPs.
- Analysis of survivin expression using immunohistochemistry and reverse transcriptase-polymerase chain reaction (RT-PCR).
- Statistical analysis including odds ratios (OR) and haplotype analysis.
Main Results:
- The C-31G CC genotype was associated with a significantly higher risk of bladder cancer (OR = 1.85).
- The A9194G G allele showed a significantly reduced risk with a gene dosage effect (OR = 0.69).
- The G-G haplotype demonstrated a significantly lower risk (OR = 0.11), indicating a cooperative effect.
- Higher nuclear survivin expression was observed in CC genotype tumors.
- Increased C-31G C allele copy number correlated with higher survivin mRNA levels.
Conclusions:
- The survivin C-31G and A9194G SNPs significantly influence bladder cancer susceptibility.
- These two SNPs exhibit a cooperative effect in modulating bladder cancer risk.
- Survivin expression levels are associated with specific genotypes of these SNPs.
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