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Published on: June 28, 2018
[Effects of human HSF1 on transcriptional regulation of prohibitin promoter]
Xiao-hua Liu1, Dong-sheng Guo, Xin-xing Wang
1Department of Stress Medicine, Institute of Health & Environmental Medicine, Tianjin 300050, China.
Aim:
To assay the transcriptional regulation effect of hHSF1 on prohibitin gene promoter.
Methods:
The total length of hHSF1 exon was amplified by PCR method and cloned into pcDNA3. 1(+) vector. pcDNA3. 1(+)-hHSF1 and pGI3 prohibitin were co-transfected into HEK293 cells. The luciferase activity was detected by Dual-Luciferase Reporter Assay System to evaluate the transcriptional regulation effect of hHSF1 on prohibitin gene promoter.
Results:
The pcDNA3.1(+)-hHSF1 vector was constructed successfully. The assay of luciferase activity showed that the transcription of pGL3-prohibitin was dramatically upregulated by hHSF1.
Conclusion:
hHSF1 can transcriptionally regulate prohibitin expression.
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