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A novel single-labeled fluorescent oligonucleotide probe for silver(I) ion detection based on the inherent quenching
Lei Wang1, Jingqi Tian, Hailong Li
1State Key Lab of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, Jilin, China.
Abstract:
A novel single-labeled fluorescent oligonucleotide (OND) probe for the detection of nanomolar silver(I) ion in aqueous solution is developed based on the inherent quenching ability of deoxyguanosines. The formation of a hairpin structure of the OND-Ag(+) complex brings deoxyguanosines close to a dye, leading to a decreased fluorescence intensity of the dye owning to photoinduced electron transfer from the dye to deoxyguanosines.
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