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Published on: January 11, 2019
[Redox dynamics in hyperthermia in rat livers by ESR spectroscopy]
Yu Cao1, Zhen Zhao, Kenichi Yamada
1Department of Physiology, China Medical University, Shenyang 110001, China.
Aim:
The redox dynamics of the rat liver in the whole body hyperthermia was measured by the decay rate of the ESR spectra used carbamoyl-PROXYL as a spin probe.
Methods:
Fifty-two Wistar male rats were randomly assigned to four groups and each group was subjected to only one of the following treatments: heat stress (HS group), anesthesia without hyperthermia (control group), pretreatment with MPG (MPG + HS group) and pretreatment with saline (No-MPG group). The solution of carbamoyl PROXYL was added to rat liver homogenate from 0, 1, 2, 6 and 24 h after heating at concentration of 0.1 mmol/L. sequentially the ESR spectra of the samples were immediately recorded by X-band ESR spectrometer.
Results:
Compared with the control group, the signal decay rate was increased gradually after heat treatment, and attained the maximum at 2 h, and then returned to the normal level at 24 h. This signal intensity effect was reduced by MPG pretreatment.
Conclusion:
Generation of reactive oxygen species in liver may be induced by heat stress.
