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Iloprost modulates the immune response in systemic sclerosis
Patrizia D'Amelio1, Maria A Cristofaro, Lucia D'Amico
1Department of Surgical and Medical Disciplines, University of Torino, Torino, Italy. patrizia.damelio@unito.it
BMC Immunology
|December 17, 2010
Summary
Iloprost, a vasodilator used for systemic sclerosis (SSc), demonstrates anti-inflammatory and immunomodulating effects by reducing TNF-alpha and T regulatory cells while increasing IL-2 and RANKL. This study investigated iloprost
Area of Science:
- Immunology and Rheumatology
Background:
- Iloprost is utilized as a vasodilator in systemic sclerosis (SSc).
- Emerging evidence suggests iloprost possesses anti-inflammatory and immunomodulating properties.
Purpose of the Study:
- To investigate the impact of iloprost on the immune response in patients with systemic sclerosis (SSc).
- To evaluate the in vivo and in vitro effects of iloprost on T cells and monocytes.
Main Methods:
- Enrolled 15 women with SSc for a 5-day iloprost infusion.
- Utilized flow cytometry, Real-time PCR, and ELISA to measure immune cell populations, gene expression, and cytokine production.
- Assessed immune responses both in vivo and in vitro.
Main Results:
- Iloprost significantly reduced T cell and TNF-alpha production.
- Observed a decrease in T regulatory cell numbers but an enhancement in their activity post-stimulation.
- Increased serum IL-2 and RANKL levels, with elevated IL-2 persisting for 28 days post-infusion; no effect on IFN-gamma.
Conclusions:
- Iloprost exhibits anti-inflammatory and immunomodulating effects in SSc patients.
- Key mechanisms include reduction of TNF-alpha and T regulatory cells, alongside augmentation of IL-2 and RANKL.
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