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Related Concept Videos

Mass Spectrum: Interpretation01:24

Mass Spectrum: Interpretation

An unknown compound can be established by identifying the molecular ion peak in the mass spectrum. The molecular ion peak is often weak or absent due to the predominance of fragmentation in high-energy electron beams. In such cases, a soft-energy electron beam can be used to scan the spectrum to enhance the intensity of the molecular ion peak. Additionally, chemical ionization, field ionization, and desorption ionization spectra are used to obtain a relatively intense molecular ion peak.To...
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
Mass Spectrometers01:16

Mass Spectrometers

This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...

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Updated: Jun 6, 2026

Sample Preparation Strategies for Mass Spectrometry Imaging of 3D Cell Culture Models
08:14

Sample Preparation Strategies for Mass Spectrometry Imaging of 3D Cell Culture Models

Published on: December 5, 2014

Perspectives in imaging using mass spectrometry.

Allison L Dill1, Livia S Eberlin, Demian R Ifa

  • 1Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA.

Chemical Communications (Cambridge, England)
|December 17, 2010
PubMed
Summary
This summary is machine-generated.

Imaging mass spectrometry (MS) provides detailed chemical information for disease diagnosis. Ambient ionization MS enables open-environment imaging, advancing forensic, clinical, and surgical applications.

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Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)
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Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)

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Optimal Preparation of Formalin Fixed Samples for Peptide Based Matrix Assisted Laser Desorption/Ionization Mass Spectrometry Imaging Workflows
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Optimal Preparation of Formalin Fixed Samples for Peptide Based Matrix Assisted Laser Desorption/Ionization Mass Spectrometry Imaging Workflows

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Last Updated: Jun 6, 2026

Sample Preparation Strategies for Mass Spectrometry Imaging of 3D Cell Culture Models
08:14

Sample Preparation Strategies for Mass Spectrometry Imaging of 3D Cell Culture Models

Published on: December 5, 2014

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)
10:47

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)

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Optimal Preparation of Formalin Fixed Samples for Peptide Based Matrix Assisted Laser Desorption/Ionization Mass Spectrometry Imaging Workflows
08:16

Optimal Preparation of Formalin Fixed Samples for Peptide Based Matrix Assisted Laser Desorption/Ionization Mass Spectrometry Imaging Workflows

Published on: January 16, 2018

Area of Science:

  • Analytical Chemistry
  • Biomedical Science
  • Forensic Science

Background:

  • Imaging mass spectrometry (MS) offers in-depth analysis of tissue chemical composition, crucial for understanding disease states.
  • Lipids and proteins are key molecular targets for diagnostic insights using MS.
  • Ambient ionization techniques have revolutionized MS by enabling analysis without complex sample preparation.

Purpose of the Study:

  • To review the historical development and evolution of imaging MS techniques.
  • To highlight the diverse applications of imaging MS in various scientific fields.
  • To underscore the potential of imaging MS for clinical diagnostics and intra-operative guidance.

Main Methods:

  • Review of historical advancements in mass spectrometry instrumentation and methodologies.
  • Discussion of ambient ionization techniques and their impact on MS imaging.
  • Compilation of current and emerging applications across forensic, clinical, and surgical settings.

Main Results:

  • Imaging MS provides high-detail molecular information for tissue analysis.
  • Ambient ionization MS simplifies the imaging process, reducing sample preparation needs.
  • The technique demonstrates significant potential for disease diagnosis and intra-surgical decision-making.

Conclusions:

  • Imaging mass spectrometry is a powerful tool for molecular tissue analysis.
  • Advancements in ambient ionization have expanded the accessibility and utility of MS imaging.
  • The integration of imaging MS into clinical practice, including surgery, is a promising future direction.