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Updated: Jun 6, 2026
Application of Mouse Parthenogenetic Haploid Embryonic Stem Cells as a Substitute of Sperm
Published on: November 19, 2020
Genetic and epigenetic X-chromosome variations in a parthenogenetic human embryonic stem cell line
Weiqiang Liu1, Yifei Yin, Yonghua Jiang
1Guangzhou Key Laboratory of Reproductive and Genetics; Institute of Gynecology and Obstetrics, The Third Affiliated Hospital of Guangzhou Medical College, Guangzhou, 510150, China.
Purpose:
To assess the genetic and epigenetic status of parthenogenetic human embryonic stem cells (phESCs).
Methods:
Cytogenetics, X chromosome inactivation (XCI) and gene expression patterns were analyzed in one phESC line (FY-phES-018) that was derived from our laboratory.
Results:
FY-phES-018 cells displayed the classical characteristics of normal hESCs. These cells had a 46, XX karyotype, and no inactive X chromosomes were observed before passage 20. After being cultured long term in vitro, some cells lost one X, and the proportion of cells with only one X gradually increased. At passage 35, almost all the cells displayed a 45, XO karyotype. Interestingly, at passage 45, the recovery of the X-chromosome was observed, and XCI became detectable; the mosaic ratio of 46, XX to 45, XO was 67:33. After passage 60, most cells displayed the 46, XX karyotype again with a mosaic ratio of 97:3. Some aberrant genomic imprinting was also observed in these cells.
Conclusions:
The phESCs line FY-phES-018 is both genetically and epigenetically unstable; therefore, further research is needed before using these cells.
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