[Detection of distinct proteins for brain injury rats serum using a protein chip]
Qing-Ming Shu1, Yong-Liang Zhang, Ling-Zhi Li
1Department of Pathology, Medical College of Chinese People's Armed Police Forces, Tianjin 300162, China.
Aim:
To explore the alteration of serum protein fingerprinting of rats subjected brain injury.
Methods:
Male Sprague-Dawley rats were randomly divided into seven groups, ie, normal control group, sham operation group, injury 4 h, 8 h, 12 h, 24 h and 48 h groups. The change of protein pattern in serum was monitored with weak cationic exchanger chip and surface-enhanced laser desorption ionization-time of flight-mass spectrometry.
Results:
The reduction of 5648 Da protein expression was detected at 4 h, 8 h or 12 h after injury (P < 0.01, compared with control and sham operation) and recovery at 24 h or 48 h. The increase of 9681 Da protein expression was detected at 4 h, 8 h or 12 h after injury (P < 0.05, compared with control and sham operation) and recovery at 24 h or 48 h.
Conclusion:
The alteration of protein expression in serum could be induced after brain injury.
