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Modeling Hypoxia/Reoxygenation Injury in Proximal Tubular Epithelial Cells
Published on: November 21, 2025
[A cell model of ischemia/reperfusion injury on skeletal muscle]
Hong-Jie Li1, Guang-Ling Zhang, Lian-Yuan Zhang
1Department of Pathophysiology of North China Coal Medical College, Tangshan 063000, China.
Aim:
To establish a model of ischemia/reperfusion injury on L-6TG cell.
Methods:
Cultured L-6TG cells were divided into 2 groups: control group (C), ischemia/reperfusion group (I/R), LDH in culture fluid, SOD, XOD, free calcium in L-6TG cell and mitochondria respiration were evaluated in each group, the micromorphologic changes were observed with microscope.
Results:
Compared with control group, after L-6TG cell suffered ischemia 4 hours and reperfusion 4 hours, LDH in culture fluid, XOD, free calcium in L-6TG cell all increased significantly, while SOD in L-6TG cell and mitochondrial respiration decreased, structural damage to L-6TG cell was severe.
Conclusion:
Using mimicking ischemic solution and mimicking reperfusion solution can successfully establish a model of ischemia/reperfusion injury on L-6TG cell.
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