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[Effects of endurance exercise on ERK1/2 phosphorylation and expression in skeletal muscle of rats]
Shi-Cheng Cao1, Li-Guang Sun, Gang Zhao
1Department of Biochemistry and Molecular Biology, China Medical University, Shenyang 110001, China.
Aim:
To investigate the effects of exercise on phosphorylated and total ERK1/2, and mRNA of ERK2.
Methods:
Male rats were randomly divided into control and trained groups. The trained rats were submitted to 1 h or 1.5 h of exercise daily and had a fragment of their excised gastroenemius muscle, 24 h or 48 h after the last training session. The train lasted for 7 weeks. The changes in the expressions of ERK1/2 and p-ERK1/2 were determined by Western blotting.The expression of ERK2 mRNA was determined by RT-PCR.
Results:
Exercise led to a marked increase in p-ERK1/2 of trained groups as compared with controls, and increased ERK1/2 protein expression of training 1.5 h/d, 24 h and 48 h after the last training session. ERK2 mRNA was increased by exercise 1 h/d, 24 h and exercise 1.5 h/d, 24 h and 48 h after the last training session. Glucose tolerance test found that blood insulin concentration was decreased with exercise training.
Conclusion:
Endurance exercise could increase muscle responsiveness to insulin by improving the total ERK1/2 and p-ERK1/2, ERK2 mRNA expression.
