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Updated: Jun 6, 2026
Isolation of Human Atrial Myocytes for Simultaneous Measurements of Ca2+ Transients and Membrane Currents
Published on: July 3, 2013
[The effect of ginkgolide B on action potential, L-type calcium current and delayed rectifier potassium current in
Xiao-Yan Qi1, Zhi-Xiong Zhang, Qi-Qi Cui
1Department of Physiology, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China.
Aim:
To study the effect of ginkgolide B from Ginkgo leave on action potential (AP), L-type calcium current (I(Ca) - L) and delayed rectifier potassium current (I(K)) in normal and ischemic guinea pig ventricular myocytes.
Methods:
With the standard microelectrode technique to record action potential and whole-cell variant patch-clamp technique to record calcium and potassium current.
Results:
(1) Under normal condition, ginkgolide B shortened APD and had no effect on RP, AP and V(max). Ginkgolide B also increased I(K) in a concentration dependent manner and had no significant effect on I(Ca) - L (2) Under ischemia condition, it was observed that shortening of APD, APA, decrease V(max) and depolarization of RP was induced by ischemia, but ginkgolide B could attenuate above--mentioned changes. (3) Under ischemia condition, I(Ca) - L and I(K) were inhibited, perfusion with ischemia solution containing ginkgolide B could reverse the decrease of I(Ca) - L and I(K).
Conclusion:
Ginkgolide B had protective effect on ischemic myocardium to prevent ischemic arrhythmia.
