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[Effects of hsf1 genotype on the constitutive expression of alphaB-crystallin in mice myocardium]
Li Liu1, Hong-Hui Zhang, Guo-Xian Ding
1Department of Gerontology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
Aim:
Try to clarify the effects of HSF1 gene on the constitutively expressed alphaBC.
Methods:
To investigate the levels of constitutively expressed alphaB-Crystallin (alphaBC) in hsf1 knockout (hsf1 -/-) and hsf1 wild type (hsf1 +/+) mice myocardium by Western blot and immunohistochemistry.
Results:
The alphaBC levels in hsf1 -/- and hsf1 +/+ were 68.42% +/- 4.16%, 100% +/- 7.58%, respectively (P < 0.05, cytosolic fraction), and 20.53% +/- 1.01%, 37.55% +/- 1.91%, respectively (P < 0.05, pellet fraction). The alphaBC signals decreased significantly in hsf1 -/- myocardium compared with hsf1 +/+ myocardium stained with fluorescence immunohistochemistry.
Conclusion:
hsf1 is the important, but not the only factor, which mediates the constitutively expressed alphaBC.
