Related Experiment Video
Updated: Jun 5, 2026
11:19
Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System
Published on: August 21, 2016
Replication patterns and organization of replication forks in Vibrio cholerae
Caroline Stokke1, Torsten Waldminghaus1, Kirsten Skarstad1
1Department of Cell Biology, Institute for Cancer Research, The Norwegian Radiumhospital, Oslo University Hospital, Oslo, Norway.
Microbiology (Reading, England)
|December 18, 2010
Summary
Vibrio cholerae replicates its two chromosomes using overlapping cycles in rich media. ChrII replication is initiated later than ChrI, with forks organizing during rapid growth for better segregation.
Area of Science:
- Microbiology
- Bacterial genetics
- Cell biology
Background:
- Vibrio cholerae possesses two chromosomes, ChrI and ChrII.
- Understanding chromosome replication is crucial for bacterial growth and division.
Purpose of the Study:
- To investigate the replication patterns of V. cholerae's two chromosomes.
- To elucidate the coordination and regulation of ChrI and ChrII replication.
Main Methods:
- Flow cytometry
- Quantitative real-time PCR
- Computer simulations
- Cell-sorting experiments
- Marker frequency analysis
- Protein synthesis inhibition assays
Main Results:
- Overlapping replication cycles observed for ChrI and ChrII in rich media.
- ChrII replication initiates after ChrI replication begins, with specific origin copy numbers in LB medium.
- Replication forks co-localize in pairs or higher-order structures, especially during rapid growth.
Conclusions:
- V. cholerae employs coordinated replication of its two chromosomes.
- Active delay in ChrII initiation facilitates ChrI replication.
- Enhanced fork organization aids in chromosome segregation during rapid growth.
More Related Videos
Related Concept Videos
01:32Replication in Prokaryotes
DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
02:35Replication in Prokaryotes
Overview
02:35Replication in Prokaryotes
Overview
01:32Prokaryotic DNA Replication
DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
01:02The DNA Replication Fork
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork. Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
01:02The DNA Replication Fork
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork. Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...