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A Flexible Chamber for Time-Lapse Live-Cell Imaging with Stimulated Raman Scattering Microscopy

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Synchronized time-lens source for coherent Raman scattering microscopy.

Ke Wang¹, Christian W Freudiger, Jennifer H Lee

¹School of Applied and Engineering Physics, Cornell University, Ithaca, New York 14853, USA. kw376@cornell.edu

|December 18, 2010

Summary

This summary is machine-generated.

We synchronized two pulsed light sources for biological imaging using a time-lens. This novel method offers a user-friendly, all-fiber alternative for coherent anti-Stokes Raman scattering (CARS) and stimulated Raman scattering (SRS) imaging.

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Area of Science:

Optics and Photonics
Biomedical Imaging
Laser Technology

Background:

Synchronizing multiple pulsed laser sources is crucial for advanced imaging techniques like CARS and SRS.
Existing synchronization methods can be complex and challenging to implement.

Purpose of the Study:

To demonstrate a new, user-friendly synchronization scheme for two pulsed light sources using the time-lens concept.
To enable synchronized dual-wavelength pulsed sources for CARS and SRS biological imaging.

Main Methods:

Utilized a time-lens concept with an all-fiber 1064 nm source.
Synchronized the time-lens source to a picosecond Ti: Sapphire mode-locked laser using its pulses as a clock.
Applied the synchronized source to CARS and SRS imaging of mouse tissues.

Main Results:

Successfully synchronized an all-fiber time-lens source to a Ti: Sapphire laser.
Demonstrated the application of the synchronized source for CARS and SRS imaging.
Achieved synchronized two-wavelength pulsed output for imaging.

Conclusions:

The time-lens approach provides an effective method for synchronizing pulsed light sources.
This all-fiber, user-friendly time-lens source is a promising alternative for future SRS imaging applications.