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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

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Related Experiment Video

Updated: Jun 5, 2026

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays
10:44

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Published on: November 13, 2017

Aptamer-based multiplexed proteomic technology for biomarker discovery.

Larry Gold1, Deborah Ayers, Jennifer Bertino

  • 1SomaLogic, Boulder, Colorado, USA. lgold@somalogic.com

Plos One
|December 18, 2010
PubMed
Summary
This summary is machine-generated.

A new aptamer-based proteomics technology enables simultaneous measurement of thousands of proteins for biomarker discovery. This method identified 58 potential chronic kidney disease (CKD) biomarkers from small samples.

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Published on: October 20, 2016

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Last Updated: Jun 5, 2026

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays
10:44

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Published on: November 13, 2017

A High Throughput, Multiplexed and Targeted Proteomic CSF Assay to Quantify Neurodegenerative Biomarkers and Apolipoprotein E Isoforms Status
07:08

A High Throughput, Multiplexed and Targeted Proteomic CSF Assay to Quantify Neurodegenerative Biomarkers and Apolipoprotein E Isoforms Status

Published on: October 20, 2016

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • High-throughput, multiplexed proteomic analysis is crucial for biological and medical research.
  • Current proteomic technologies face challenges in efficiency and scale.

Purpose of the Study:

  • To develop and validate a novel aptamer-based proteomic technology for efficient biomarker discovery.
  • To demonstrate the technology's utility in identifying disease-specific protein signatures.

Main Methods:

  • Developed a new generation of aptamers with chemically modified nucleotides for enhanced library diversity.
  • Created an assay that measures thousands of proteins from small sample volumes (15 µL serum/plasma).
  • Quantified protein concentrations by transforming them into DNA aptamer concentrations detected on a DNA microarray.

Main Results:

  • The assay measures 813 proteins with a median limit of detection of 1 pM and a dynamic range of ~100 fM-1 µM.
  • Achieved a median coefficient of variation of 5% for protein measurements.
  • In a chronic kidney disease (CKD) study, identified 2 known and 58 potential CKD biomarkers.

Conclusions:

  • Introduced a versatile, highly multiplexed tool for large-scale proteome profiling and biomarker discovery.
  • This technology facilitates unbiased identification of novel biomarkers, advancing evidence-based medicine.
  • The aptamer-based approach aids in discovering unique protein signatures for various disease states.