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[Distribution and efficiency of pEGFP-N1 plasmid after its transfection in cardiomyocytes]
Hong Feng1, Wen-lin Xiu, Rui Zhan
1Institute of Health and Environmental Medicine, Academy of Military Medical Sciences, Tianjin 300050, China.
Aim:
To study the distribution and efficiency of pEGFP-N1 plasmid after its transfection in cardiomyocytes.
Methods:
The neonatal rat cardiomyocytes were cultured. According to the different grow period of neonatal rat cardiomyocytes, the distribution and efficiency of pEGFP-N1 plasmid after its transfection in cardiomyocytes were studied.
Results:
The efficiency was significantly increased after pEGFP-N1 plasmid transfection in one-day cardiomyocytes. The distribution of EGFP was in the cytoplasm and nucleus.
Conclusion:
The efficiency of pEGFP-N1 plasmid after its transfection in cardiomyocytes was related to the grow period of neonatal rat cardiomyocytes. The distribution of EGFP was in the cytoplasm and nucleus.
