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[Effects of oxygen-glucose deprivation on cultured rat hippocampal neurons]
Ai-Shi Ding1, Yah Wu, Zi-Min Ma
1Department of Neurobiology, Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Bejing 100850, China.
Aim:
To investigate the effects of oxygen-glucose deprivation on cultured rat hippocampal neurons.
Methods:
The hippocampal neurons cultured for 12 d were exposed to combined oxygen-glucose deprivation for 0.5 - 4 h and then cultured with original medium in normoxia for 28 h. Necrotic neurons were identified by 0.4% trypan blue staining and apoptotic neurons were detected by a TUNEL technique. Meanwhile, the area, perimeter and circle diameter of cell bodies were measured respectively by a photography analysis system.
Results:
The percentage of necrotic cells in cultured hippocampal neurons increased significantly during oxygen-glucose deprivation, but the percentage of apoptotic cells increased significantly after 28 h oxygen-glucose recovery. Photography analysis showed that area, perimeter and circle diameter of the necrotic cell bodies were larger than those of the apoptotic ones.
Conclusion:
Oxygen-glucose deprivation can lead to severe damage of cultured hippocampal neurons. The necrosis is major during acute oxygen-glucose deprivation, while the apoptosis is major 28 h after oxygen-glucose recovery.
