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Related Experiment Video

Updated: Jun 5, 2026

Murine Model of CD40-activation of B cells

Murine Model of CD40-activation of B cells

Published on: March 5, 2010

Microvascular thrombosis and CD40/CD40L signaling.

F N E Gavins¹, G Li, J Russell

¹Wolfson Neuroscience Laboratories, Faculty of Medicine, Imperial College, London, UK. f.gavins@imperial.ac.uk

Journal of Thrombosis and Haemostasis : JTH

|December 21, 2010

Summary

This summary is machine-generated.

The CD40/CD40L signaling system plays a role in inflammation-enhanced thrombosis. This system contributes to microvascular thrombosis in both brain and muscle, with varying effects depending on the vessel type and location.

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Murine Model of CD40-activation of B cells

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Detection of CD40 Protein-Umbelliferone Interaction via Differential Scanning Fluorescence

Detection of CD40 Protein-Umbelliferone Interaction via Differential Scanning Fluorescence

Published on: March 1, 2024

Area of Science:

Immunology
Vascular Biology
Thrombosis Research

Background:

Inflammation and thrombosis are interconnected processes.
The molecular links between inflammation and thrombosis are not fully understood.
The CD40/CD40L signaling pathway is a potential mediator.

Purpose of the Study:

To investigate the role of the CD40/CD40L system in inflammation-induced microvascular thrombosis.
To assess this role in experimental models of endotoxemia (lipopolysaccharide) and colitis (dextran sodium sulfate).

Main Methods:

Utilized wild-type (WT), CD40-deficient, and CD40L-deficient mice.
Induced thrombosis in cerebral and cremaster muscle arterioles and venules using light/dye photoactivation.
Administered soluble CD40L to CD40L-deficient mice in the LPS model.

Main Results:

CD40 and/or CD40L deficiency impacted inflammation-enhanced thrombosis in both cerebral and muscle vasculatures.
The specific contributions of CD40 and CD40L varied between vascular beds and vessel types.
Soluble CD40L administration partially reversed the protective effect of CD40L deficiency in cerebral arterioles.

Conclusions:

The CD40/CD40L pathway is implicated in enhanced thrombus formation during acute and chronic inflammation.
Differential roles of CD40 and CD40L exist in various vascular beds and vessel types.
Targeting the CD40/CD40L system may offer therapeutic potential for inflammatory thrombotic conditions.