[Protoplast reversion in Bacillus subtilis]

The reversion of the protoplasts of two strains Bac. subtilis on 3 nutritive media: DP of Elliott et al., DM3 of Chang and Cohen and MRG of Bourne and Dancer, is studied. Special attention is devoted to the role of the components gelatin, bovine serum albumin and osmotic stabilizer. In relation to the conclusions drawn in the study a new medium for reversion of protoplasts is suggested--the glucitole reversion agar GRA ensuring continuous 20-40% reversion of the sown protoplasts.