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Are antibiotic screening approaches sufficiently adequate? A proficiency test
Bjorn J A Berendsen1, Mariël G Pikkemaat, Linda A A M Stolker
1RIKILT, Wageningen University and Research Centre, Akkermaalsbos 2, 6708WB, P.O. Box 230, 6700AE Wageningen, The Netherlands. bjorn.berendsen@wur.nl
A 2009 proficiency test revealed significant shortcomings in antibiotic screening methods for beef. Instrumental methods showed the lowest false negative rates, highlighting the need for improved screening approaches and regular proficiency testing.
Area of Science:
- Food safety analysis
- Veterinary drug residues
Background:
- Antibiotic residues in beef pose a food safety concern.
- Effective screening methods are crucial for monitoring these residues.
Purpose of the Study:
- To evaluate the suitability of cryogenically minced beef for proficiency testing.
- To assess the performance of various antibiotic screening methods used by laboratories.
Main Methods:
- A proficiency test was conducted using blank and spiked beef samples (flumequine, lincomycin/spectinomycin).
- Cryogenically minced materials underwent homogeneity and stability studies.
- Participants utilized in-house screening methods (microbial, biochemical, instrumental).
Main Results:
- Cryogenically minced spiked muscle material is suitable for antibiotic residue analysis.
- False negative rates varied significantly: 73% (microbial), 50% (biochemical), and 22% (instrumental).
Conclusions:
- Substantial improvement is needed in current antibiotic screening approaches for beef.
- Regular proficiency tests are essential to identify and address deficiencies in screening methods.
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