Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
SDS-PAGE01:27

SDS-PAGE

Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...
Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as  cells...
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cationic separation of medium strong bases by electrophoretic focusing on inverse electromigration dispersion profile. Sensitive analysis of beta-blockers in blood and water with ESI-MS detection.

Journal of chromatography. A·2025
Same author

Recent progress in analytical capillary isotachophoresis (2018 - March 2022).

Journal of chromatography. A·2022
Same author

Cationic electrophoretic focusing on inverse electromigration dispersion profile with ESI-MS detection. New capillary electrophoretic method for high-sensitivity analysis of 2-hydroxy-s-triazines in waters.

Analytica chimica acta·2022
Same author

At-line coupling of hollow fiber liquid-phase microextraction to capillary electrophoresis for trace determination of acidic drugs in complex samples.

Talanta·2021
Same author

System eigenmobilities in zone electrophoresis: A general moving-boundary approach.

Electrophoresis·2021
Same author

Capillary isotachophoresis with electrospray-ionization mass-spectrometric detection: Cationic electrolyte systems in the medium-alkaline range for selective analysis of medium strong bases.

Journal of chromatography. A·2020

Related Experiment Video

Updated: Jun 5, 2026

Electrophoretic Separation of Proteins
08:17

Electrophoretic Separation of Proteins

Published on: June 12, 2008

Contemporary sample stacking in analytical electrophoresis.

Zdena Malá1, Petr Gebauer, Petr Boček

  • 1Institute of Analytical Chemistry of the Academy of Sciences of the Czech Republic, Brno, Czech Republic.

Electrophoresis
|December 21, 2010
PubMed
Summary

This review surveys recent advancements in sample stacking techniques for capillary electrophoresis (CE), crucial for enhancing analytical sensitivity. It covers key methods like Kohlrausch adjustment, pH steps, and micellar systems across various applications.

More Related Videos

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
09:35

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling

Published on: April 1, 2017

Analysis of Mitochondrial Respiratory Chain Complexes in Cultured Human Cells using Blue Native Polyacrylamide Gel Electrophoresis and Immunoblotting
07:55

Analysis of Mitochondrial Respiratory Chain Complexes in Cultured Human Cells using Blue Native Polyacrylamide Gel Electrophoresis and Immunoblotting

Published on: February 12, 2019

Related Experiment Videos

Last Updated: Jun 5, 2026

Electrophoretic Separation of Proteins
08:17

Electrophoretic Separation of Proteins

Published on: June 12, 2008

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
09:35

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling

Published on: April 1, 2017

Analysis of Mitochondrial Respiratory Chain Complexes in Cultured Human Cells using Blue Native Polyacrylamide Gel Electrophoresis and Immunoblotting
07:55

Analysis of Mitochondrial Respiratory Chain Complexes in Cultured Human Cells using Blue Native Polyacrylamide Gel Electrophoresis and Immunoblotting

Published on: February 12, 2019

Area of Science:

  • Analytical Chemistry
  • Separation Science

Background:

  • Sample stacking is essential for achieving high sensitivity in capillary electrophoresis (CE) analyses.
  • Regular literature surveys are valuable for experts and practitioners in the field.
  • This work continues a series of reviews on sample stacking techniques.

Purpose of the Study:

  • To provide a comprehensive overview of recent developments in sample stacking for CE.
  • To organize and present the latest research findings in the field over the past two years.

Main Methods:

  • Literature review covering the last two years.
  • Categorization of techniques based on their underlying principles: Kohlrausch adjustment of concentrations, pH step, micellar systems, and combined techniques.
  • Sorting of literature by application fields such as clinical, pharmaceutical, food, and environmental analysis.

Main Results:

  • Identification and summarization of novel sample stacking strategies published recently.
  • Organization of findings into distinct methodological sections for clarity.
  • Coverage of diverse application areas demonstrating the versatility of sample stacking.

Conclusions:

  • Sample stacking remains a critical area of research in CE for improving analytical performance.
  • The reviewed techniques offer significant potential for enhanced sensitivity in various analytical domains.
  • Continued research in sample stacking is vital for advancing analytical capabilities in diverse scientific fields.