[Quantitative identification Qijudihuang pill by integrating overall information method based on parallel five...

Parallel five wavelength high performance liquid chromatographic (PFW-HPLC) fingerprints of Qijudihuang Pill (QJDHP) were established. The quality of QJDHP was identified based on systematically quantified fingerprint method (SQFM) by the integrating overall information method (IOIM). The chromatographic fingerprints (CFPs) were developed by reversed-phase (RP) HPLC, in which 51, 49, 52, 49 and 47 co-existing peaks were marked at 203, 228, 265, 280 and 326 nm, respectively, by choosing paeonol (POL) peak as the reference peak. The natural weighted method (NWM), average method (AM) and project parameter method (PPM) were separately used to integrate the different chemical qualitative and quantitative information from different PFW-HPLC fingerprints. By using the IOIM based on SQFM, the qualities of DMS2, DMS4, DMS5 and DMS6 (2 grade) were identified as very good; DMS1, DMS3 and DMS10, DMS11 (3 grade) as good; DMS9 (4 grade) as fine; DMS7 (6 grade) as common, in which DMS8 was regarded as moderate (5 grade) by the AM and as common (6 grade) by PPM. The qualities of 11 batches of QJDHP were identified, in which 8 batches were above the good level, 1 batch was fine level and two batches were common level. Therefore AM was the most accurate and simple method to integrate all the information. The experimental results indicated that the IOIM based on the PFW-HPLC can be effectively used to identify qualitatively and quantitatively the quality of Chinese traditional medicine from overall information, which is a reliable method and also can quantitatively simplify the bountiful information of HPLC-DAD (diode array detection) fingerprints. In fact, the comprehensive identification ability of IOIM is the most reliable and effective one.