[Determination of esculeoside A in Lycopersicon esculentum MILL by high performance liquid chromatography with
Fenglai Lu1, Jinlei Liu, Yueyuan Chen
1Guangxi Institute of Botany, Chinese Academy of Sciences, Guilin 541006, China.
Abstract:
A method for the determination of esculeoside A in Lycopersicon esculentum MILL by high performance liquid chromatography (HPLC) with evaporative light scattering detection (ELSD) was established. The results showed that esculeoside A had good linearity in the range of 0.61-3.05 mg with the correlation coefficient of 0. 999 5. The average recoveries were 97.9%-104.8% with the relative standard deviations (RSD) < or = 4.14% (n=5). The method is simple, sensitive and suitable for the analysis of esculeoside A and quality control for the raw material and extract of Lycopersicon esculentum MILL.
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