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Production and Purification of Non Replicative Canine Adenovirus Type 2 Derived Vectors
Published on: December 3, 2013
[Construction of antisense recombinant adenoviral vector for c-myc and its antiproliferative effect on rat
Ying-ge Liu1, Hao-wen Qi, Huan-zhang Li
1Department of Respiratory Medicine, Xijing Hospital, Fourth Military Medical University, Xi' an 710033, China.
Aim:
To observe the antiproliferative effect of antisense recombinant adenoviral vector for c-myc on rat thymus lymphocytes.
Methods:
Antisense and sense bacterial plasmids for c-myc were constructed. Bacterial plasmids and El detected adenoviral plasmid were cotransfected into 293 cells. Recombinant adenoviral vectors were obtained after cotransfection. The antiproliferative effects were assayed by MTS. The expression of c-myc mRNA was detected by RT-PCR.
Results:
The results showed that antisense recombinant adenoviral vector for c-myc could inhibit rat thymus lymphocytes proliferation. The expression of c-myc mRNA was decreased after antisense recombinant adenoviral vector for c-myc was transfected into cells.
Conclusion:
Recombinant antisense adenoviral vector for c-myc could inhibit rat thymus lymphocytes proliferation.
