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Published on: November 8, 2011
[Cloning of B7-1 and B7-2 genes from human Burkitt's B lymphocyte line]
1Department of Immunology, Affiliated Hospital for Academy of Medical Sciences, Beijing 100039, China.
Aim:
In order to construct recombinant B7-Pseudomonas exotoxin fusion protein to induce long-term immunotolerance, we tried to clone both B7-1 and B7-2 genes from human Burkitt's B lymphocyte line (Raji) and construct the recombinant plasmids encoding for B7-1 and B7-2.
Methods:
Two pairs of primers for B7-1 and B7-2 were designed and synthesized according to the sequences o human B7-1 and B7-2 genes derived from genbank. Two recombinant plasmids pGEM-T-B7-1 land pGEM-T-B7-2 were constructed by recombinant gene techniques.
Results And Conclusion:
We have successfully cloned both B7-1 and B7-2 cDNA-which, confirmed by DNA sequencing, EcoRI/Hind III and BamHI/Sph1 restriction enzyme digesting, could be used to construct recombinant B7-Pseudomonas exotoxin fusion proteins.
