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Updated: Jun 5, 2026
A Neuronal and Astrocyte Co-Culture Assay for High Content Analysis of Neurotoxicity
Published on: May 5, 2009
[The protective effect of puerarin on cultured rat cerebral cortical astrocytes]
Aim:
To study the effects of puerarin (Pue) on impairment of rat astrocytes in primary cell culture induced by oxygen-glucose deprivation (OGD) or sodium glutamate (Glu).
Methods:
Astrocyte damage induced by (OGD), Glu or (+/-)-1-aminocyclopentane-trans-1,3-dicarboxylic acid (trans-ACPD), as well as the action of Pue was measured by determining the intracellular water space (as measured by 3-O-methyl-[1- 3H]D-glucose uptake) of astrocytes and the leakage of lactate dehydrogenase (LDH) from astrocytes.
Results:
Following the exposure to OGD for 5 h, 0.5 mmol/L Glu or 1 mmol/L trans-ACPD for 1 h, the astrocyte volume and LDH leakage from astrocytes were increased. 0.1 mmol/L Pue, when co-incubated with OGD, Glu or trans-ACPD, reduced astrocytic swelling and the LDH leakage.
Conclusion:
Pue had protective effects on astrocytes damaged by OGD, Glu or trans-ACPD.
