Related Experiment Video
Updated: Jun 5, 2026
Isolation and Genetic Manipulation of Adult Cardiac Myocytes for Confocal Imaging
Published on: September 17, 2009
[The study of transfected cardiomyocytes by recombinant adenovirus and adeno-associated virus]
Aim And Methods:
Recombinant adenovirus (rAd) and adeno-associated virus (rAAV) were created, in which beta2-adrenergic receptors (beta2-AR) gene is under control of the cmv promotor, the cultured neonate rat ventricular myocytes were infected by the two vectors, and the expression of beta2-AR on cultured neonate rat ventricular myocytes was assessed.
Results:
RT-PCR demonstrated the presence of beta2-AR mRNA, protein immunoblots demonstrated the expression of the beta2-AR gene. According to a ligand binding assay, the density of beta AR in the cardiomyocytes infected by rAd and rAAV had no difference, which was greater than that in the control.
Conclusion:
The results above demonstrated that adenovirus vector and AAV vector transfected efficiently cardiomyocytes.
