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Updated: Jun 5, 2026
Comparative Analysis of Human Growth Hormone in Serum Using SPRi, Nano-SPRi and ELISA Assays
Published on: January 7, 2016
[The radioimmunoassay of growth hormone and prolactin and its application]
1Department of Neurobiology and Physiology, College of Life Sciences, Zhejiang University, Hangzhou 310027, China.
Aim:
To set up a highly specific and sensitive double antibody radioimmunoassay (RIA) for determining the contents of growth hormone (GH) and prolactin (PRL) in pituitary and plasma of rats; To study the effects of acute hypoxia on GH and PRL of rats.
Methods:
We use chloramine-T technique iodinate antigen and choose the equilibration and saturated procedure of RIA to add the preparations.
Results:
Exposed to 7 km-hypoxia for 0.5 h, pituitary GH content increased (P < 0.05) and conversely, plasma GH levels were suppressed (P < 0.05); while the PRL levels in both pituitary and plasma were suppressed (P < 0.01); and there was no significantly difference between 5 km hypoxia groups and control.
Conclusion:
We have successfully established the method of radiolabel and double antibody RIA with highly sensitivity; acute hypoxia (7 km) suppressed the secretion of GH and PRL in rats.
