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Updated: Jun 5, 2026
Cell Type-specific Gene Expression Profiling in the Mouse Liver
Published on: September 17, 2019
[Isolation of specific expression gene in human fetal liver by representational difference analysis]
Aim:
To isolate specific gene expressed in human fetal liver tissue and find out genes with important biological functions.
Methods:
mRNA obtained from 4-months human fetus liver tissue was used as tester, and mRNA obtained from adult liver tissue was used as driver, and representational difference analysis (RDA) was performed. The subtracted final product was subcloned into the pGEM-T easy vector, after transformation, bacterial colonies were randomly picked, and 54 plasmid clones with inserts were purified and sequenced. The homology search of genes was performed by online-based BLAST program through the nonredundant and EST database at Nation Center of Biotechnology Information (NCBI), the expression pattern of novel gene was further verified by competitive PCR.
Results:
Marker with alpha-globin gene family, its expression frequency in subtractive cDNA pool is 7 times than non-subtractive cDNA library, and the pool contains many genes closely relative with liver growth. These show that RDA is an effective method to isolate gene differential expression. Through sequencing some clones, we get two sequences not reported before.
Conclusion:
The library is a useful means to study genes expressed specially in fetal liver.
