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Updated: Jun 5, 2026

Visualization of Neutrophil Extracellular Traps in Mesenteric Venules After Mesenteric Ischemia-Reperfusion Injury via Intravital Microscopy
07:05

Visualization of Neutrophil Extracellular Traps in Mesenteric Venules After Mesenteric Ischemia-Reperfusion Injury via Intravital Microscopy

Published on: September 27, 2024

[Vacuole formation and endothelial damage in microvessels after ischemia reperfusion]

Y Y Liu1, X M Zhao, F Y Liu

  • 1Laboratory of Microcirculaiton PLA General Hospital, Beijing 100853.

Zhongguo Ying Yong Sheng Li Xue Za Zhi = Zhongguo Yingyong Shenglixue Zazhi = Chinese Journal of Applied Physiology
|December 22, 2010
PubMed
Abstract

Insights

Ischemia reperfusion in rat mesentery microvessels causes endothelial cell edema and vacuole formation. Leukocyte and platelet adhesion also occurs, indicating serious vascular injury.

Area of Science:

  • Physiology
  • Pathology
  • Microcirculation

Context:

  • Ischemia reperfusion injury is a significant clinical concern.
  • Understanding microvascular changes is crucial for developing therapeutic strategies.

Purpose:

  • To investigate endothelial cell damage and the adhesion of leukocytes and platelets in rat mesentery microvessels following ischemia reperfusion.
  • To characterize the morphological changes in microvessels post-reperfusion.

Summary:

  • Rat mesentery microvessels were subjected to ischemia followed by reperfusion.
  • Microscopic observation revealed leukocyte and platelet adhesion in venules and capillaries within 1-3 hours.
  • Endothelial cells exhibited edema, vascular wall thickening, and intracytoplasmic vacuole formation, particularly prominent in arterioles.

Impact:

  • The findings demonstrate significant endothelial cell injury, evidenced by edema and vacuole formation, following ischemia reperfusion.
  • These cellular changes highlight the vulnerability of microvasculature to ischemic events.
  • The study provides insights into the early cellular mechanisms of ischemia reperfusion injury in microvessels.

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