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[Expression of ΔNp73 in human NSCLC and clinical implication]
Yong He1, Shizhi Fan, Yaoguang Jiang
1Thoracic Surgery Center of People's Liberation Army, Daping Hospital/The Research Institute of Surgery, The Third Military Medical University, Chongqing 400042, P.R.China.
Background:
ΔNp73 is an isoform of the p53 homologue p73, which lacks an NH₂-terminal transactivation domain and antagonizes the induction of gene expression by p53 and p73. The aim of this study is to investigate the expression of ΔNp73 mRNA in non-small cell lung cancer (NSCLC) and to analyse its relations with clinicopathologic factors and prognosis.
Methods:
Semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR) was used to detect the expression of ΔNp73 mRNA in 40 resected NSCLC specimens with the neighboring noncancerous tissue. The significance of ΔNp73 mRNA expression was analyzed statistically.
Results:
ΔNp73 mRNA was highly expressed in lung cancer tissues (62.7%, 32/51) while negative in neighboring noncancerous tissue. The expression of ΔNp73 mRNA was associated with pathological TNM stage (P=0.046), but not associated with age, gender, histological type and differentiation status. Survival of patients with high ΔNp73 mRNA was significantly poorer than those with low ΔNp73 mRNA levels (P < 0.001). Multivariate analysis revealed that ΔNp73 mRNA levels were a significant prognostic factor, independent of the other conventional prognostic factors.
Conclusions:
NSCLC has overexpression of ΔNp73 mRNA, which is closely related to TNM stages and prognosis of patients with NSCLC. These results suggest that measurement of ΔNp73 mRNA levels in tumor tissues might be useful as a promising predictor for the prognosis of patients with NSCLC.
Insights
Overexpression of ΔNp73 mRNA in non-small cell lung cancer (NSCLC) correlates with advanced TNM stages and poorer patient prognosis. Measuring ΔNp73 mRNA may predict NSCLC outcomes.
Area of Science:
- Molecular oncology
- Cancer biology
- Gene expression analysis
Context:
- ΔNp73, a p53 homologue isoform lacking a transactivation domain, antagonizes p53 and p73 functions.
- Non-small cell lung cancer (NSCLC) is a major cause of cancer-related mortality worldwide.
- Understanding novel molecular markers is crucial for improving NSCLC diagnosis and prognosis.
Purpose:
- To investigate the expression levels of ΔNp73 mRNA in NSCLC tissues.
- To analyze the relationship between ΔNp73 mRNA expression and clinicopathological factors in NSCLC.
- To evaluate the prognostic significance of ΔNp73 mRNA in NSCLC patients.
Summary:
- Semiquantitative RT-PCR detected significantly higher ΔNp73 mRNA expression in NSCLC tissues (62.7%) compared to adjacent noncancerous tissues.
- ΔNp73 mRNA expression was associated with pathological TNM stage (P=0.046) but not with age, gender, histology, or differentiation.
- High ΔNp73 mRNA levels correlated with significantly poorer patient survival (P < 0.001) and were an independent prognostic factor.
Impact:
- ΔNp73 mRNA is overexpressed in NSCLC and linked to TNM staging.
- ΔNp73 mRNA levels serve as a significant independent prognostic factor for NSCLC.
- Quantification of ΔNp73 mRNA in tumor tissue shows potential as a predictive biomarker for NSCLC prognosis.
