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Updated: Jun 5, 2026

Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays
Published on: September 26, 2011
Abbott RealTime HIV-1 m2000rt viral load testing: manual extraction versus the automated m2000sp extraction
Lesley E Scott1, John A Crump, Emma Msuya
1University of the Witwatersrand, Department of Molecular Medicine and Haematology, School of Pathology, Faculty of Health Science, 7 York Rd Parktown, Johannesburg 2000, South Africa. lesley.scott@nhls.ac.za
None:
The Abbott RealTime HIV-1 assay is a real-time nucleic acid amplification assay available for HIV-1 viral load quantitation. The assay has a platform for automated extraction of viral RNA from plasma or dried blood spot samples, and an amplification platform with real time fluorescent detection. Overall, this study found no clinically relevant differences in viral load, if samples were extracted manually.

