[Determination of aromadendrin in Euonymus alatus by HPLC]
Yunhua Chen1, Muxin Gong, Chao Zhang
1College of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China. wscyh9759@sina.com
Objective:
To establish a HPLC method to determine the content of aromadendrin in Euonymus alatus.
Method:
Kromasil 100-5C18 column was used with a mobile phase composed of acetonitrile-0.3% glacial acetic acid (24:76) at a flow rate of 1.0 mL x min(-1). The detection wavelength was 292 nm and the temperature of column was 30 degrees C.
Result:
The calibration curve was linear in the range of 0.051-2.04 microg for aromadendrin. The correlation coefficient of the calibration curve was 0.999 9. Twenty batches of the crude E. alatus purchased from different areas were determined and the contents of aromadendrin in the twigs of E. alatus were fluctuated from 0.001 57% to 0.041 6%.
Conclusion:
This method is simple, repeatable and can be used for determination of aromadendrin.
