Related Experiment Video
Updated: Jun 5, 2026
Strategies for Study of Neuroprotection from Cold-preconditioning
Published on: September 2, 2010
[Cold stress induces the suppression of splenic NK cell activity and the c-fos expression in rat brain]
Xing-hong Jiang1, Shi-yu Guo, Shuang Xu
1Laboratory of Neurobiology, School of Medicine, Suzhou University, Suzhou 215007, China.
Aim:
To observe the effect of cold stress on the splenic NK cell activity and the c-fos expression in rat brain.
Methods:
Rats were maintained in cold chamber at 4 degrees for 4 h. The 51Cr release assay from YAC-1 cells was used to determine the splenic NK cell activity and the double staining of ABC method was employed to observe the immunoreactive expression of Fos, arginine-vasopressin and tyrosine hydroxylase.
Results:
Cold stress could induce a marked suppression of splenic NK cell activity and a significant expression of Fos protein in hypothalamic paraventricular nucleus(PVN), as well as in the brainstem locus coeruleus (LC). Double staining showed c-expression of Fos and arginine-vasopressin in some of PVN neurons, and co-expression of Fos and tyrosine hydroxylase in the majority of LC neurons.
Conclusion:
The vasopressinergic neurons in PVN and the catecholaminergic neurons in LC might be probably involved in the suppression of splenic NK cell activity induced by cold stress.
More Related Videos
09:12Assessing Cellular Stress and Inflammation in Discrete Oxytocin-secreting Brain Nuclei in the Neonatal Rat Before and After First Colostrum Feeding
Published on: November 14, 2018
07:26Assessment of Stress Effects on Cognitive Flexibility using an Operant Strategy Shifting Paradigm
Published on: May 4, 2020