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Characterization of a chemically synthesized RNA having the sequence of the yeast initiator tRNA(Met)
J Bratty1, T F Wu, K Nicoghosian
1Département de Biochimie, Université de Montréal, Qué, Canada.
FEBS Letters
|August 20, 1990
Abstract:
A 75-unit long oligoribonucleotide corresponding to the sequence of the Saccharomyces cerevisiae initiator tRNA was synthesized chemically. The crude RNA was purified, and the sequence was verified by RNA sequencing techniques. A particularly useful purification step involved hydrophobic chromatography on BND-cellulose. The purified RNA could be aminoacylated to 28% of a bona fide initiator tRNA(Met) sample and threonylated to 76% of the level observed with native tRNA(fMet) from E. coli.