Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Inhibition of Cdk Activity

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...

Inhibition of CDK Activity

Inhibition of CDK Activity

No description available

Positive Regulator Molecules

Positive Regulator Molecules

Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.

Positive Regulator Molecules

Positive Regulator Molecules

To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.

M-Cdk Drives Transition Into Mitosis

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...

M-Cdk Drives Transition Into Mitosis

M-Cdk Drives Transition Into Mitosis

No description available

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Phosphatase specificity influences phosphorylation timing of CDK substrates during the cell cycle.

Nature communications·2025

Same author

Spatiotemporal orchestration of mitosis by cyclin-dependent kinase.

Nature·2025

Same author

Science, Education, Leadership, and Politics: An Interview with Bruce Alberts and Paul Nurse.

Annual review of cell and developmental biology·2025

Same author

High-Throughput Measurement of Single-Fission Yeast Cell Volume Using Fluorescence Exclusion.

Methods in molecular biology (Clifton, N.J.)·2024

Same author

The discovery of cyclin-dependent kinases.

Nature reviews. Molecular cell biology·2024

Same author

We can make the UK a science superpower - with a radical political manifesto.

Nature·2024

Search research articles

Related Experiment Video

Updated: Mar 31, 2026

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

Driving the cell cycle with a minimal CDK control network.

Damien Coudreuse¹, Paul Nurse

¹Laboratory of Yeast Genetics and Cell Biology, The Rockefeller University, 1230 York Avenue, New York, New York 10065, USA. dcoudreuse@rockefeller.edu

|December 24, 2010

Summary

This summary is machine-generated.

Researchers created a simplified cell cycle control system in fission yeast. This minimal network, driven by a single cyclin-dependent protein kinase (CDK) oscillator, efficiently regulates cell reproduction and division.

More Related Videos

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Published on: June 6, 2017

Experimental Approaches to Study Mitochondrial Localization and Function of a Nuclear Cell Cycle Kinase, Cdk1

Experimental Approaches to Study Mitochondrial Localization and Function of a Nuclear Cell Cycle Kinase, Cdk1

Published on: February 25, 2016

Related Experiment Videos

Last Updated: Mar 31, 2026

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Published on: June 6, 2017

Experimental Approaches to Study Mitochondrial Localization and Function of a Nuclear Cell Cycle Kinase, Cdk1

Experimental Approaches to Study Mitochondrial Localization and Function of a Nuclear Cell Cycle Kinase, Cdk1

Published on: February 25, 2016

Area of Science:

Cell Biology
Molecular Biology
Systems Biology

Background:

Eukaryotic cell proliferation is governed by complex regulatory networks.
Understanding the fundamental principles of the cell cycle remains challenging due to this complexity.

Purpose of the Study:

To investigate the core regulatory engine of the mitotic cell cycle.
To construct a minimal control network that sustains cellular reproduction in fission yeast.

Main Methods:

Engineered a minimal, monomolecular cyclin-dependent protein kinase (CDK) module in fission yeast.
Removed much of the canonical regulatory components from the CDK module.
Observed the behavior of the engineered network to assess cell cycle progression.

Main Results:

The engineered CDK module oscillates, driving orderly progression through major cell cycle events.
This CDK oscillator acts as the primary organizer of the cell cycle.
Two CDK activity thresholds were identified, defining independent cell cycle phases and imposing timing and directionality.

Conclusions:

A simple, core architecture based on a CDK oscillator can form the basic control of the eukaryotic cell cycle.
This minimal network efficiently sustains cellular reproduction.
The findings simplify our understanding of cell cycle regulation.