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Porous Substrate-Based Electroporation with Transepithelial Electrical Impedance Monitoring
Published on: September 27, 2024
Evaluation of delivery conditions for cutaneous plasmid electrotransfer using a multielectrode array
B Ferraro1, L C Heller, Y L Cruz
1Department of Molecular Medicine, University of South Florida, Tampa, FL, USA.
Gene Therapy
|December 24, 2010
Summary
A novel multielectrode array (MEA) enables efficient in vivo electroporation (EP) for large-area skin DNA delivery. This method allows for controlled transgene expression by adjusting voltage and plasmid dose.
Area of Science:
- Biomedical Engineering
- Molecular Biology
- Dermatology
Background:
- Electroporation (EP) is a method for delivering molecules into cells.
- Current EP methods face challenges in large-surface area tissue delivery.
- A multielectrode array (MEA) offers a potential solution for enhanced delivery.
Purpose of the Study:
- To evaluate a multielectrode array (MEA) for in vivo electroporation (EP).
- To assess the impact of field strength and pulse width on transgene expression.
- To determine the viability of MEA for cutaneous DNA delivery.
Main Methods:
- Utilized a guinea pig model for intradermal plasmid DNA injection.
- Applied EP with a novel MEA design to the skin.
- Measured luciferase gene expression to quantify delivery efficiency.
Main Results:
- Increased voltage magnitude and duration correlated with higher luciferase expression.
- Transgene expression levels were controllable via field strength and plasmid dose.
- The MEA facilitated efficient plasmid DNA delivery to large skin areas.
Conclusions:
- The MEA design is effective for cutaneous DNA delivery via in vivo EP.
- This technology allows for tunable transgene expression.
- MEA-based EP is a promising approach for large-surface area genetic material delivery to skin.
