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[Effects of CGRP on LPS-induced MMP-9 secretion by alveolar macrophages]
Yong-ping Liu1, Cha-xiang Guan, Hong-bo Bai
1Department of Physiology, Xiang Ya Medical School, Central South University, Changsha 410078, China.
Aim:
To explore the effects of calcitonin-gene-related peptide (CGRP) on LPS-induced MMP-9 secretion by alveolar macrophages (AM) in vitro.
Methods:
The supernatant of LPS-induced Wistar rat AM from different intervention groups were collected to measure the activity by gelatin zymography.
Results:
(Only secreting a small amount of MMP-9 with unstimulated AM, LPS stimulated MMP-9 production in a concentration-dependent manner (p < 0.01). (2) The activity of MMP-9 in CGRP intervention groups at different levels were significantly lower than those in non-intervention group (p < 0.01). (3) The inhibiting effects of CGRP were diminished by H-7 and W-7, an antagonist of protein kinase C (PKC) and calmodulin (CaM) (p < 0.05).
Conclusion:
These data suggested that CGRP involved in the MMP-9 secretion by AM, partly, via PKC and CaM pathway.
