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Updated: Jun 5, 2026
A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices
Published on: August 11, 2021
[The research on monoamine neurotransmitters changes in plasma and cerebrospinal fluid in patients with persistent
Xue-Fang Hu1, Pei-Dong Wang, De-Gon Yang
1Department of Biochemistry, Second Military Medical University Nanjing Military Medical College, Nanjing 210099, China.
Aim:
To study the relationships between the pathogenesis of persistent vegetative state (PVS) and the levels of monoamine neurotransmitters in plasmas and cerebrospinal fluids (CSFs) in patients with PVS.
Methods:
The high-pressure liquid chromatography (HPLC) was used to detect.
Results:
It was found that the level of dopamine (1.95 +/- 0.99) was significantly increased in plasma compared with the control (1.16 +/- 0.47) (P < 0.05), but the levels of 5-HT, TYR, TRP, GABA of plasma were insignificantly changed. In cerebrospinal fluid, the level of DA was insignificantly changed, and the level of 5-HT (0.49 +/- 0.32) was significantly decreased compared with the control (1.02 +/- 0.35) (P < 0.05), but the levels of TYR (1.36 +/- 0.11), TRP (0.63 +/- 0.40), GABA (1.15 +/- 0.61) were significantly increased respectively compared with the controls (0.40 +/- 0.24; 0.29 +/- 0.22; 0.37 +/- 0.45) (P < 0.05; P < 0.05; P < 0.01). The levels of GABA and DA in plasma were significant correlated (P < 0.05), and GABA and DA in CSF were also significant correlated (P < 0.01).
Conclusion:
The results of this study suggested that PVS is related to the decrease of 5-HT in CSF and the increase of DA in plasma.
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