Related Experiment Video
Updated: Jun 5, 2026
Study of Viral Vectors in a Three-dimensional Liver Model Repopulated with the Human Hepatocellular Carcinoma Cell Line HepG2
Published on: October 24, 2016
[Study on construction of a plasmid vector carrying human hepatocyte growth factor gene and activity of its
Xiao-Qin Ha1, Xin-Guo Wang, Zu-Ze Wu
1Institute of Radiation Medcine, Academy of Military Medical Sciences, Beijing 100850, China.
Aim:
To construct a plasmid carrying hepatocyte growth factor gene and investigate its effects in vitro.
Methods:
A complementary DNA (cDNA) clone for human HGF was isolated from human placental cDNA, then subcloned into pUDK vector, which was constructed by ourselves, to form the pUDKH plasmid. The transfection efficiency and the expression level of HGF and VEGF were evaluated by transfecting pUDK or pUDKH into primary rat skeletal muscle cells. The biological effects of HGF-expressing product at different doses on endothelial cells were investigated in vitro, and assessed by MTT.
Results:
The primary rat skeletal muscle cells could be transfected efficiently with pUDKH (0.057%), and secreted HGF(16 -18 ng/4 x 10(5) cells) and VEGF proteins. The expressing product could significantly stimulate proliferation of human umbilical vein endothelial cells, in a dose-dependent manner (P < 0.05).
Conclusion:
pUDKH has the potential application in vivo to treat ischemic diseases.
More Related Videos
10:18Generation of Plasmid Vectors Expressing FLAG-tagged Proteins Under the Regulation of Human Elongation Factor-1α Promoter Using Gibson Assembly
Published on: February 9, 2015
09:35Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle
Published on: February 1, 2017