Related Experiment Video
Updated: Jun 5, 2026
IgY Technology: Extraction of Chicken Antibodies from Egg Yolk by Polyethylene Glycol (PEG) Precipitation
Published on: May 1, 2011
[Comparison between anti-ouabain egg yolk(IgY) and rabbit antibody(IgG) in enzyme-linked immunosorbent assay]
Ming-juan Zhang1, Jun Yang, Heng Ge
1Department of Cardiology, Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710004, China.
Aim:
To improve specificity and accuracy of endogenous ouabain measurement assay.
Methods:
Anti-ouabain polyclonal antibody egg yolk (IgY) and anti-ouabain rabbit antibody (IgG) were prepared respectively. In the presence of two kinds of antibody, then the specificity and accuracy of enzyme-linked immunosorbent assay (ELISA) were compared.
Results:
The ELISA, in the presence of IgY, provided a sensitivity of the average intraassay coefficient of variation(CV) was 2.03%, and the inter-assay CV was 2.34% respectively. In contrast, IgG were 2.83% and 3.29%. No significant interferences were observed with hydrocortisone and dexamethasone. There was 3.45% vs. 5.95%, 3.20% vs. 5.20% of crossreaction with cedilanid and digoxin.
Conclusion:
The specificity and accuracy of ELISA, in which IgY was used, were more better than IgG.
