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Published on: August 1, 2013
Characterization of Suid herpesvirus 1 field isolates from Argentina
M S Serena1, G E Metz, G P Martín Ocampos
1Cátedra de Virología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, 60 y 118, CC 296 (1900) La Plata, Argentina. solserena2000@fcv.unlp.edu.ar
Abstract:
The genomic characterization of Suid herpesvirus 1 (SHV-1) isolates from Argentina was accomplished by restriction pattern analysis using the BamHI, BstEII and XhoI enzymes. Type II genome has been described only once in Argentina. This study revealed considerable homogeneity of BamHI endonuclease sites in all the strains analyzed, according to the number and size of the fragments. No deletion of BamHI fragment #7 among the Argentinean isolates suggests that these strains are wild-type. In addition, the main antigenic domain of glycoprotein E of all the Argentinean strains, as well as the reference strains and sequences available in the GenBank, were characterized. The similarity percent oscillated between 99 and 100%.
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